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Bradykinin inhibits a potassium M‐like current in rat pheochromocytoma PC12 cells
Author(s) -
Villarroel Alvaro,
Marrion Neil V.,
Lopez Hector,
Adams Paul R.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81057-9
Subject(s) - bradykinin , pheochromocytoma , potassium , endocrinology , medicine , chemistry , pharmacology , biology , receptor , organic chemistry
We studied the action of bradykinin (BK) on ionic currents in fused pheochromocytoma PC12 cells under voltage‐clamp in whole‐cell mode, and on intracellular calcium using fura‐2. BK induced the development of an outward current associated with an increase in intracellular calcium, followed by inhibition of an M‐like current. The outward current was blocked by (+)‐tubocurarine, and prevented when the calcium chelator BAPTA or high concentrations of inositol 1,4,5‐triphosphate were introduced into the cell, whereas the M‐like current and its inhibition by BK remained unaffected. The protein kinase activator phorbol 12,13 dibutyrate partially reduced the M‐current. M‐current density did not substantially change after prolonged treatment with nerve growth factor.