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Molecular cloning and sequence analysis of cDNA encoding rat adrenal cytochrome P‐450 11β
Author(s) -
aka Yasuki,
Matsukawa Naomichi,
Morohashi Ken-ichirou,
Omura Tsuneo,
Ogihara Toshio,
Teraoka Hiroshi,
Okamoto Mitsuhiro
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81053-1
Subject(s) - complementary dna , peptide sequence , nucleic acid sequence , amino acid , biology , microbiology and biotechnology , open reading frame , cytochrome , molecular cloning , protein primary structure , biochemistry , gene , enzyme
A cDNA clone encoding cytochrome P‐450 11β of rat adrenal has been cloned and sequenced using a bovine P‐450 11β cDNA insert (pcP‐450(11β)‐2; (1987) J. Biochem. 102, 559–568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P‐450 11β precursor protein consisting of 499 amino acids including an extension peptide of 24 amino acids at the NH 2 ‐terminus. The cDNA contains 1247 nucleotides at the 3′‐noncoding region including 51 nucleotides of poly A, but lacks the 5′‐noncoding region. The deduced amino acid sequence shows 61% similarity to that of bovine P‐450 11β . Putative binding sites for heme and steroid are highly conserved among steroidogenic P‐450s of known structure.