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AVP receptors of mouse Leydig cells are regulated by LH and E 2 and influenced by experimental cryptorchidism
Author(s) -
Tahri-Joutei Abderrafih,
Pointis Georges
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81036-1
Subject(s) - leydig cell , endocrinology , medicine , testosterone (patch) , receptor , stimulation , chemistry , testicle , biology , hormone , luteinizing hormone
Exposure of pubertal mouse Leydig cells for 24 h to increasing concentrations (1–100 ng/ml) of LH elicited a dose‐dependent decrease in AVP receptor content. Maximal reduction (50%) was obtained at a dose of 10 ng/ml LH. A similar treatment applied to adult Leydig cells did not influence AVP receptor density. Treatment of adult Leydig cells for 24 h by E 2 (5–500 ng/ml) resulted in a dose‐dependent increase in AVP receptor content. About 50% increase was achieved with 500 ng/ml E 2 . AVP receptor content in pubertal Leydig cells was not modified irrespective of the concentration of E 2 tested. These changes in AVP receptor number were well correlated with the response of Leydig cells to AVP (10 −6 M) in terms of testosterone production. 2 weeks bilateral cryptorchidism resulted in reduction of testicular weight, circulating testosterone levels associated with a marked rise in Leydig cell AVP receptor density with no change of affinity. Testosterone production by Leydig cells from cryptorchid testes in response to AVP (10 −6 M) or hCG (100 ng/ml) stimulation was reduced compared to that of control Leydig cells. This study provides new arguments supporting the concept that AVP could be involved in local regulation of testicular steroidogenesis.