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Measurement of cromakalim‐induced 87 Rb flux in intact cells by NMR
Author(s) -
Williamson Michael P.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81032-4
Subject(s) - cromakalim , extracellular , intracellular , chemistry , efflux , reagent , biophysics , potassium , intracellular ph , nuclear magnetic resonance , biochemistry , biology , physics , agonist , receptor , organic chemistry
87 Rb nuclear magnetic resonance signals from intracellular and extracellular Rb + can be distinguished by the use of shift reagents, thus permitting the simultaneous measurement of intracellular and extracellular Rb concentrations. When smooth muscle cells are suspended in a medium containing Rb + in place of K + , there is a slow influx of Rb + , which is greatly increased by the addition of cromakalim to the medium. The concentration of cromakalim required to stimulate influx is similar to that needed to stimulate 86 Rb efflux from isolated tissue.