Premium
Interactions of formylmethionyl‐leucyl‐phenylalanine, adenosine, and phosphodiesterase inhibitors in human monocytes Effects on superoxide release, inositol phosphates and cAMP
Author(s) -
Elliott Keith R.F.,
Leonard Edward J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)81016-6
Subject(s) - chemistry , inositol , phosphodiesterase , superoxide , adenosine , phenylalanine , biochemistry , pharmacology , enzyme , biology , receptor , amino acid
Cessation of the fMLF‐induced burst of human monocyte superoxide release was associated with a rise in cAMP. This was not due to inhibition of phophodiesterase (PDE), the major form of which was the PDE IV isozyme. The action of burst inhibitors did not correlate with cAMP levels: Rolipram, a PDe IV inhibitor, increased cAMP 6‐fold, with minimal effects on the burst; whereas theophylline increased cAMP less than 2‐fold but decreased the burst to less than half. Although theophylline and the adenylate cyclase activator, adenosine, inhibited fMLF‐induced superoxide release, they did not inhibit production of inositol phosphates. Thus, these studies on inhibition of superoxide release implicated neither cAMP nor inositol phosphates.