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Diameter of the mitochondrial outer membrane channel: Evidence from electron microscopy of frozen‐hydrated membrane crystals
Author(s) -
Mannella Carmen A.,
Guo Xiao-Wei,
Cog Bernard
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80965-2
Subject(s) - membrane , electron microscope , barrel (horology) , bacterial outer membrane , biophysics , chemistry , inner mitochondrial membrane , electron , crystallography , analytical chemistry (journal) , materials science , optics , biology , physics , chromatography , biochemistry , composite material , escherichia coli , quantum mechanics , gene
The average projected density of mitochondrial outer membrane channels has been obtained from electron microscopic images of unstained, frozen‐hydrated two‐dimensional crystals. The density map is consistent with the projection of hollow, thin‐walled cylinders aligned normal to the membrane plane with a diameter of 3.8 ± 0.1 nm. This diameter closely matches that of the largest β‐barrel that can be formed from one copy of the 30‐kDa mitochondrial outer membrane channel protein.