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Gene transfer in plant protoplasts Inhibition of gene activity by cytosine methylation and expression of single‐stranded DNA constructs
Author(s) -
Hershkovitz Maty,
Gruenbaum Yosef,
Zakai Nehama,
Loyter Abraham
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80952-4
Subject(s) - chloramphenicol acetyltransferase , protoplast , transfection , biology , gene , dna methylation , microbiology and biotechnology , dna , gene expression , plasmid , reporter gene , petunia , genetics
Transfection of petunia protoplasts with the plasmid pUC118CaMVCAT, bearing the bacterial chloramphenicol acetyltransferase (CAT) gene, resulted in the appearance of CAT activity. No CAT activity was detected in protoplasts transfected with the hemimethylated form of pUC118CaMVCAT. Transfection of petunia protoplasts with ss DNA constructs bearing the coding and noncoding sequences of the CAT gene also resulted in the appearance of CAT activity, indicating expression of ss DNA constructs in transfected protoplasts. The relevance of our observations to control of gene expression in plant cells is discussed.