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Identification of Nsp100 as elongation factor 2 (EF‐2)
Author(s) -
Koizumi Shinichi,
Ryazanov Alexey,
Hama Tokiko,
Chen Hao-Chia,
Guroff Gordon
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80928-7
Subject(s) - elongation factor , eukaryotic translation elongation factor 1 alpha 1 , protein kinase a , phosphorylation , phosphoprotein , kinase , elongation , calmodulin , chemistry , microbiology and biotechnology , biochemistry , biology , enzyme , ribosome , materials science , rna , ultimate tensile strength , metallurgy , gene
The nerve growth factor‐sensitive phosphoprotein from PC12 cells, previously designated Nsp100, has been shown to be elongation factor 2 (EF‐2). The criteria used for this identification include: (i) similarity of N‐terminal sequence; (ii) phosphorylation by the same kinase; (iii) ADP‐ribosylation mediated by diphtheria toxin; (iv) comparable function in cell‐free protein synthesis. According to these criteria, Nsp 100 and EF‐2 are identical and the kinase that phosphorylates Nsp100 in PC12 cells is calcium/calmodulin kinase III.