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Na + /H + exchange is not necessary for protein kinase C‐mediated effects in platelets
Author(s) -
Krishnamurthi Sushila,
Patel Yatin,
Morgan Winston A.,
Wheeler-Jones Caroline P.D.,
Kakkar Vijay V.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80908-1
Subject(s) - protein kinase c , chemistry , platelet , extracellular , protein kinase a , platelet activation , granule (geology) , phorbol , phosphorylation , biophysics , membrane , biochemistry , medicine , biology , paleontology
The role of Na + /H + exchange in protein kinase C‐mediated effects in platelets was investigated by studying the effect of removal of extracellular Na + ([Na + ] e ) on the different responses induced by phorbol 12‐myristate 13‐acetate (PMA) and 1,2‐ dioctanoylglycerol (diC 8 ). None of the responses studied, namely, protein phosphorylation, translocation of enzyme activity to the membrane fraction, potentiatory and inhibitory effects on platelet activation ([Ca 2+ ] i , arachidonate and granule release) showed an absolute dependence on [Na + ] e . With the exception of dense‐granule release, which was clearly potentiated by the removal of [Na + ] e and showed a negative correlation with exchanger activity, the other effects of PMA and diC 8 were not affected by [Na + ] e removal. It is concluded that Na + /H + exchange is not essential for protein kinase C activation in platelets.