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The narK gene product participates in nitrate transport induced in Escherichia coli nitrate‐respiring cells
Author(s) -
Noji Sumihare,
Nohno Tsutomu,
Saito Taiichi,
Taniguchi Shigehiko
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80906-8
Subject(s) - operon , escherichia coli , nitrate , mutant , gene , gene product , biochemistry , plasmid , peptide sequence , biology , nucleic acid sequence , chemistry , gene expression , ecology
The nucleotide sequence of the Escherichia coli narK gene, which is located in the upstream region of the narCHJI operon, was determined. The narK gene encodes a very hydrophobic protein with 463 amino acid residues ( M r 49 693). A narK deletion mutant, under conditions for the induction of nitrate respiration, was unable to perform nitrate transport. Loss of transport activity was recovered by transforming the mutant with a narK + plasmid. Thus, we conclude that the narK gene encodes a transmembrane protein participating in nitrate transport. In the narK promoter region, we defined a unique sequence that we designate as a ‘nitrate box’, functioning as a putative NarL‐binding site, in addition to the consensus sequence of the ‘anaero‐box’.