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Radiation inactivation analysis of the A 1 adenosine receptor of rat brain Decrease in radiation inactivation size in the presence of guanine nucleotide
Author(s) -
Reddington Martin,
Klotz Karl-Norbert,
Lohse Martin J.,
Hietel Bernhard
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80903-2
Subject(s) - guanine , adenosine , gtp' , chemistry , nucleotide , g protein , photoaffinity labeling , protein subunit , ligand (biochemistry) , biochemistry , receptor , adenosine receptor , adenosine a1 receptor , affinity label , biophysics , biology , agonist , enzyme , gene
Radiation inactivation analysis of the binding of the A 1 adenosine receptor antagonist, 8‐cyclopentyl‐1,3‐dipropylxanthine to rat brain membranes yielded a radiation inactivation size of 58 kDa. In the presence of GTPγS this was reduced to 33 kDa, in good agreement with the size of the ligand‐binding subunit detected after photoaffinity labelling. The data indicate that the structural association of A 1 adenosine receptors with G‐protein components is altered in situ in the presence of guanine nucleotides.