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Synthesis and properties of tachyplesin I, a lipopolysaccharide‐binding peptide, from Tachypleus tridentatus
Author(s) -
Shieh Tiee-Cherng,
Kohzuma Takuji,
Park Nam Gyu,
Ohno Motonori,
Nakamura Takanori,
Iwanaga Sadaaki,
Yamamoto Tatsuto
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80902-0
Subject(s) - chemistry , peptide , biochemistry
Tachyplesin I, a cationic heptadecapeptide amide containing two disulfide bonds isolated from hemocytes of Tachypleus tridentatus , which binds to lipopolysaccharide, was synthesized by a solid‐phase procedure. Reduction and oxidation of the deprotected peptide amide yielded an identical peptide to native tachyplesin I in terms of chemical and biological properties. Tachyplesin 1 was found to bind to alkylphosphorylcholine accompanied by perturbation of its tyrosyl and tryptophyl residues but bound only weakly to N ‐acetyl‐D‐glucosamine tetramer.