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Methylation of elongation factor EF‐Tu affects the rate of trypsin degradation and tRNA‐dependent GTP hydrolysis
Author(s) -
Toledo Héctor,
Jerez Carlos A.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80885-3
Subject(s) - ethionine , gtp' , methylation , elongation factor , ef tu , gtpase , chemistry , biochemistry , escherichia coli , trypsin , transfer rna , elongation , microbiology and biotechnology , biology , rna , enzyme , methionine , amino acid , dna , ribosome , ultimate tensile strength , metallurgy , gene , materials science
The role of methylation of elongation factor EF‐Tu from Escherichia coli was investigated. The methylated factor was obtained from cells harvested at the late stationary growth phase. Submethylated forms of the factor were obtained from either bacteria grown to the mid‐logarithmic phase or cells cultured in the presence of ethionine. When fully methylated EF‐Tu was treated in the presence of trypsin, it showed a greatly reduced rate of degradation compared with both types of undermethylated EF‐Tu factors. The methylation of EF‐Tu appeared not to affect the rate of GDP and GTP exchange. However, aminoacyl‐tRNA‐stimulated GTPase activity determined in the presence of kirromycin was greatly increased when the EF‐Tu was methylated.