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Characterization of Mg 2+ efflux from human, rat and chicken erythrocytes
Author(s) -
Günther T.,
Vormann J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80812-9
Subject(s) - efflux , chemistry , choline , sucrose , sodium , biochemistry , nuclear chemistry , chromatography , organic chemistry
Net Mg 2+ efflux from Mg 2+ ‐loaded human, rat and chicken erythrocytes was measured in sucrose, NaCl and choline Cl medium. Thus, Na + ‐dependent (NaCl minus choline Cl) and Na + ‐independent Mg 2+ efflux (in sucrose) were determined. Na + ‐dependent Mg 2+ efflux amounted to 0.16, 8.9 and 1.57 mmol/l cells × 30 min, Na + ‐independent Mg 2+ efflux amounted to 0.89, 1.55 and 0.37 mmol/l cells × 30 min for human, rat and chicken erythrocytes. Na + ‐dependent Mg 2+ efflux was inhibited by quinidine. Na + ‐independent Mg 2+ efflux was inhibited by SITS and Cl − . A small fraction of Na + ‐independent Mg 2+ efflux (in choline Cl) was resistant to SITS and Cl − . Ca 2+ loading increased Mg 2+ efflux similar to K + efflux (Gardos effect). This effect was differently expressed in human and chicken erythrocytes.