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Quantitative immunochemical evidence for identical topography of subunits CF 0 II and CF 0 I within the photosynthetic ATP‐synthase of spinach chloroplasts
Author(s) -
Otto Joachim,
Berzborn Richard J.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80810-5
Subject(s) - spinach , antiserum , chemistry , chloroplast , polyclonal antibodies , atp synthase , biochemistry , thylakoid , photosynthesis , agglutination (biology) , microbiology and biotechnology , biology , antibody , enzyme , gene , genetics
Monospecific polyclonal antisera against the 16 kDa and the 18 kDa polypeptides II and I from the CF 0 moiety of the photosynthetic ATP‐synthase in spinach were produced. Inhibition, agglutination and absorption experiments were combined with quantitative determinations in ELISA of residual antibodies and of amounts of CF 0 and CF 1 . It follows that subunits CF 0 II and CF 0 I are membrane anchored, extend into the matrix space, and are partially shielded underneath or within CF 1 . These results on the structure suggest that CF 0 II and CF 0 I are homologous to each other, and in function both homologous to E. coli F 0 b; thus CF 0 II together with CF 0 I would be part of the ‘stalk’.