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Basic fibroblast growth factor enhances the capacity of bone marrow cells to form bone‐like nodules in vitro
Author(s) -
Noff Dina,
Pitaru Sandu,
Savion Naphtali
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80808-7
Subject(s) - basic fibroblast growth factor , osteoblast , alkaline phosphatase , bone marrow , in vitro , chemistry , endocrinology , fibroblast growth factor , medicine , growth factor , thymidine , microbiology and biotechnology , biology , biochemistry , enzyme , receptor
The role of basic fibroblast growth factor (bFGF) in the proliferation and differentiation of rat bone marrow cells in culture was studied. bFGF stimulated [ 3 H]thymidine incorporation into these cells by 4‐fold at a concentration of 0.3 ng/ml and half‐maximal effect was observed at a concentration of 15 pg/ml. In addition to its mitogenic effect, bFGF stimulated alkaline phosphatase activity by 3.6‐fold. Continuous treatment with bFGF (for 21 days) resulted in a 6.3‐fold increase in the culture dish surface area covered by bone‐like mineralized tissue. Maximal bone‐like tissue formation was observed in the presence of 3 ng/ml bFGF with half‐maximal effect at a concentration of 0.3 ng/ml. These results indicate the possible role of bFGF in the proliferation of osteogenic rat bone marrow cells and their differentiation into cells of osteoblast‐like phenotype.