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An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues
Author(s) -
Cohen Philip,
Klumpp Susanne,
Schelling Donald L.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80803-8
Subject(s) - phosphatase , biochemistry , chemistry , computational biology , biology , phosphorylation
The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1–2 nMadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 μMadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.