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Isolation of cDNA clones encoding the human Sm B/B′auto‐immune antigen and specifically reacting with human anti‐Sm auto‐immune sera
Author(s) -
Sharpe N.G.,
Williams D.G.,
Howarth D.N.,
Coles B.,
Latchman D.S.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80801-4
Subject(s) - complementary dna , clone (java method) , antigen , cdna library , biology , microbiology and biotechnology , antibody , immune system , immunology , gene , genetics
A cDNA clone for the human SmB and B′ auto‐immune antigens has been isolated by antibody screening of a cDNA expression library. The cDNA clone hybridises with two distinct mRNAs, one of which is expressed in a tissue‐specific manner. A fusion protein expressed from the cDNA clone was recognised by a number of sera from systemic lupus erythematosus (SLE) patients containing anti‐Sm antibodies but not by sera reactive with other auto‐immune antigens. The potential use of this clone in a diagnostic assay for SLE and in elucidating the processes regulating the expression of SmB and B′ is discussed.