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A cDNA clone encoding the precursor for a 10.2 kDa photosystem I polypeptide of barley
Author(s) -
Okkels Jens Sigurd,
Scheller Henrik Vibe,
Jepsen Lars Bæk,
Møller Birger Lindberg
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80799-9
Subject(s) - complementary dna , open reading frame , molecular mass , biology , peptide sequence , photosystem i , microbiology and biotechnology , photosystem ii , nucleic acid sequence , protein primary structure , coding region , biochemistry , hordeum vulgare , gene , photosynthesis , enzyme , botany , poaceae
Two cDNA clones for the barley photosystem I polypeptide which migrates with an apparent molecular mass of 9.5 kDa on SDS‐polyacrylamide gels have been isolated using antibodies and an oligonucleotide probe. The determined N‐terminal amino acid sequence for the mature polypeptide confirms the identification of the clones. The 644 base‐pair sequence of one of the clones contains one large open reading frame coding for a 14 882 Da precursor polypeptide. The molecular mass of the mature polypeptide is 10 193 Da. The hydropathy plot of the polypeptide shows one membrane‐spanning region with a predicted α‐helix secondary structure. The gene for the 9.5 kDa polypeptide has been designated PsaH .