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Import of a mutant mitochondrial precursor fails to respond to stimulation by a cytosolic factor
Author(s) -
Randall Stephen K.,
Shore Gordon C.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80796-3
Subject(s) - reticulocyte , mitochondrion , cytosol , mutant , lysis , biochemistry , stimulation , chemistry , microbiology and biotechnology , amino acid , biology , enzyme , gene , messenger rna , endocrinology
Import of the precursor to ornithine carbamyltransferase is stimulated by a partially‐purified, NEM‐sensitive soluble factor from rabbit reticulocyte lysate. A mutant in which the carboxy‐terminal 73 amino acids were deleted, had a sharply reduced response to this factor. The NEM‐sensitive, import‐stimulating factor interacts with the surface of mitochondria in the absence of precursor protein. Thus reticulocyte lysate contains an NEM‐sensitive, import stimulating factor which interacts both with the surface of mitochondria and whose activity appears to be dependent upon the structure of the mature portion of the precursor.