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Immunodetection of Na,K‐ATPase α3‐isoform in renal and nerve tissues
Author(s) -
Arystarkhova E.A.,
Lakhtina O.E.,
Modyanov N.N.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80793-8
Subject(s) - gene isoform , protein subunit , biochemistry , atpase , chemistry , nephron , peptide sequence , peptide , microbiology and biotechnology , biology , enzyme , gene , renal function
At least three types of mRNA of the catalytic subunit of Na,K‐ATPase namely α‐,α+‐ and α3‐isoforms are identified in different tissues. Only two of them α and α+ have well known structural and catalytic properties. Here we present immunochemical data indicating that the α3 protein really exists in pig and human kidney, and human brain. Crude membrane fractions and purified membrane‐bound Na,K‐ATPases were immunoblotted with α3‐specific antibodies raised against the synthetic peptide corresponding to the unique sequence of this isoform. The mature α3‐subunit is shown to include the sequence GDKKDDKSSPK followed by the initiating methionine residue. Nephron collecting tubules are proposed to specifically contain Na,K‐ATPase α3‐isoform.