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Synergistic enhancement of type I and III collagen production in cultured fibroblasts by transforming growth factor‐β and ascorbate
Author(s) -
Appling W.Douglas,
O'Brien Wanda R.,
Johnston Dennis A.,
Duvic Madeleine
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80792-6
Subject(s) - fibronectin , extracellular matrix , transforming growth factor , wound healing , transforming growth factor beta , chemistry , cell growth , type i collagen , fibroblast , angiogenesis , fibrosis , growth factor , type iv collagen , microbiology and biotechnology , ascorbic acid , in vitro , biochemistry , biology , immunology , cancer research , endocrinology , laminin , medicine , receptor , food science
Transforming growth factor‐β (TGF‐β) is a prototype of a family of polypeptides that regulates cellular growth and phenotypic differentiation [(1986) Science 233, 532‐534; (1987) Cell 49, 437‐438]. TGF‐β injection induces angiogenesis and fibrosis locally [(1986) Proc. Natl. Acad. Sci. USA 83, 4167‐4171; (1987) Science 237, 1333‐1336] and stimulates the synthesis of extracellular matrix proteins, fibronectin, collagens, and proteoglycans in vitro in many cell types [(1986) J. Biol. Chem. 261, 4337‐4345; (1987) Biochem J. 247, 597‐604]. Ascorbate is also known to induce collagen synthesis and to promote wound healing [(1988) J. Invest. Dermatol. 90, 420‐424; (1986) Coll. Rel. Res. 6, 455‐466]. We report that in cultured human skin fibroblasts, ascorbate and TGF‐β synergistically enhance the biosynthesis of type I and III collagens and their steady‐state mRNAs. TGF‐β alone has no enhancing effect on type III collagen synthesis. The cooperation between ascorbate and TGF‐β may be of significance in wound healing and in disorders of fibrosis.