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α‐ and β‐tubulin mRNAs of Trypanosoma cruzi originate from a single multicistronic transcript
Author(s) -
Soares Célia M.A.,
de Carvalho Elizeu F.,
Urményi Turán P.,
O. Carvalho José Francisco,
de Castro Firmino T.,
Rondinelli Edson
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80784-7
Subject(s) - tubulin , biology , complementary dna , trypanosoma cruzi , rna , microbiology and biotechnology , gene , nuclease , dna , genome , genetics , microtubule , parasite hosting , computer science , world wide web
The cluster of alternated α‐ and β‐tubulin genes in the genome of Trypanosoma cruzi was shown to be transcribed into a single RNA molecule which upon processing gives rise to the mature α‐ and β‐tubulin mRNAs. This conclusion was based on: (i) nuclear RNA species with the same molecular mass hybridize to both α‐ and β‐tubulin cDNA probes; (ii) S 1 nuclease assay of the clustered tubulin genes has shown protected DNA fragments of the same size and of greater molecular mass than that corresponding to the mRNAs, hybridizable to both α‐ and β‐tubulin cDNA probes; (iii) β‐tubulin hybrid selected RNA is still able to hybridize to α‐tubulin probe.