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Characterization of cDNA clones encoding two putative isoforms of the α 1 subunit of the dihydropyridine‐sensitive voltage‐dependent calcium channel isolated from rat brain and rat aorta
Author(s) -
Koch Walter J.,
Hui Anna,
Shull Gary E.,
Ellinor Patrick,
Schwartz Arnold
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80761-6
Subject(s) - gene isoform , complementary dna , dihydropyridine , protein subunit , calcium channel , voltage dependent calcium channel , calcium , chemistry , microbiology and biotechnology , biochemistry , biophysics , biology , gene , organic chemistry
cDNA clones encoding rat brain and rat aorta isoforms of the α 1 subunit of the dihydropyridine‐sensitive, voltage‐dependent calcium channel were isolated and sequenced. These tissue‐specific cDNA clones share significant amino acid similarity with the rabbit skeletal muscle calcium channel α 1 subunit (75% and 66% amino acid identity for rat brain and rat aorta isoforms, respectively). Northern analysis revealed transcript sizes of 6.5 and 8.6 kb in aorta and 8.6 kb in brain.