Premium
Receptor‐independent metabolism of platelet‐activating factor by myelogenous cells
Author(s) -
O'Flaherty Joseph T.,
Chabot Marie C.,
Redman Jimmy,
Jacobson David,
Wykle Robert L.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80751-3
Subject(s) - platelet activating factor , receptor , platelet activating factor receptor , pronase , chemistry , antagonist , receptor antagonist , platelet , biochemistry , microbiology and biotechnology , pharmacology , biology , endocrinology , immunology , enzyme , trypsin
Human neutrophils incorporate and metabolize platelet‐activating factor (PAF). We dissociated these events from PAF binding to its receptors. Cells were pretreated with either pronase, a PAF antagonist (L652731), or excess PAF. This reduced PAF receptor numbers by 70 to almost 100% but had no comparable effect upon the neutrophil's ability to metabolize PAF. Furthermore, HL‐60 cells efficiently metabolized, but did not specifically bind, PAF. Thus, PAF receptor availability did not correlate with PAF metabolic capacity and we conclude that myelogenous tissues can process this bioactive ligand by a receptor‐independent pathway.