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Potentiation by adrenaline of thrombin‐induced elevation of pH i is not essential for synergistic activation of human platelets
Author(s) -
Steen Vidar M.,
Cook Carol A.,
Tysnes Ole-Bjørn,
Holmsen Holm
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80722-7
Subject(s) - thrombin , platelet , chemistry , long term potentiation , platelet activation , biophysics , cytoplasm , medicine , endocrinology , biochemistry , receptor , biology
Gel‐filtrated human platelets were stimulated with thrombin in the absence and presence of adrenaline. Adrenaline markedly enhanced the thrombin‐induced increase in cytoplasmic pH (pH i ) in BCECF‐loaded platelets. This rise in pH i was strongly inhibited by the Na + /H + exchange blocker EIPA. The potentiation by adrenaline of thrombin‐induced PLC activation measured as [ 32 P]PA formation and final platelet responses was, however, not blocked by EIPA, even at low concentrations of thrombin. These results indicate that the enhancement by adrenaline of thrombin‐induced cytoplasmic alkalinization may be a secondary effect which is not essential for the potentiation by adrenaline of platelet activation by thrombin.