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Elucidation of multiple forms of nephrocalcin by 31 P‐NMR spectrometer
Author(s) -
Nakagawa Y.,
Otsuki T.,
Coe F.L.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80717-3
Subject(s) - chemistry , phosphate , chromatography , oxalate , cellulose , nuclear magnetic resonance spectroscopy , calcium oxalate , calcium , dissociation (chemistry) , residue (chemistry) , phosphatase , nuclear chemistry , enzyme , stereochemistry , biochemistry , inorganic chemistry , organic chemistry
Four forms of nephrocalcin have been routinely isolated from mammalian kidney tissues and urine using DEAE‐cellulose column chromatography with a linear NaCl gradient. We have demonstrated that these four forms of nephrocalcin, isolated from bovine kidneys, contain different amounts of phosphate residues, and that alkaline phosphatase digestion converts these to only one form of nephrocalcin. The changes in the nephrocalcin before and after removal of phosphate residues were measured by 31 P‐NMR spectrometer. Loss of phosphate residues decreased the dissociation constant of nephrocalcin 10‐fold toward calcium oxalate monohydrate crystals, suggesting the phosphate residues appear to be important in the inhibitory effects of calcium oxalate monohydrate crystal growth.