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Lectin‐like proteins accumulate as fragmentation products in bean seed protein bodies
Author(s) -
Ceriotti Aldo,
Vitale Alessandro,
Bollini Roberto
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80710-0
Subject(s) - endoplasmic reticulum , biology , lectin , phaseolus , fragmentation (computing) , escherichia coli , fusion protein , biochemistry , cotyledon , microbiology and biotechnology , gene , botany , recombinant dna , ecology
The gene sequence of the Phaseolus vulgaris L. seed lectin‐like protein (LLP) and its precursor (∼ 40 kDa) which is associated with the endoplasmic reticulum has been described, while the molecular characteristics of the mature protein and its cellular localization are still unknown. Our data, obtained using antibodies raised against a fusion protein, which was produced in Escherichia coli and contained most of the LLP sequence, indicate that mature LLP accumulates in the protein bodies of cotyledon cells. LLP consists of several polypeptides in the M r range 15 000 to 20 000, which are a result of proteolytic fragmentation of the protein precursor.