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3′‐Fluoro‐3′‐deoxyribonucleoside 5′‐triphosphates: Synthesis and use as terminators of RNA biosynthesis
Author(s) -
Mikhailopulo Igor A.,
Pricota Tamara I.,
Poopeiko Nicolai E.,
Sivets Grigorii G.,
Kvasyuk Eugenii I.,
Sviryaeva Tatiana V.,
Savochkina Larissa P.,
Beabealashvilli Robert S.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80706-9
Subject(s) - deoxyribonucleoside , biosynthesis , chemistry , rna , biochemistry , dna , gene
3′‐Fluoro‐3′‐deoxy‐uridine, ‐cytidine, ‐adenosine and ‐guanosine have been synthesized by glycosylation of the corresponding silylated bases with 1‐ O ‐acetyl‐2,5‐di‐ O ‐benzoyl‐3‐fluoro‐3‐deoxy‐D‐ribofuranose in the presence of Friedel‐Crafts catalysts and were converted to the 5′‐ triphosphates, NTP(3′‐F). It was shown that NTP(3′‐F) are terminators of RNA synthesis catalyzed by DNA‐dependent RNA polymerase from E. coli and may thus serve as tools for DNA sequencing.