Premium
Modulation of DNA methyltransferase during the life cycle of a mealybug Planococcus lilacinus
Author(s) -
Devajyothi C.,
Brahmachari Vani
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80705-7
Subject(s) - mealybug , methyltransferase , instar , biology , dna methyltransferase , dna , enzyme , biochemistry , botany , larva , methylation , pest analysis
The levels of de novo DNA methyltransferase were studied during the development of a mealybug, Planococcus lilacinus . No enzyme activity could be detected in extracts from second instar females. But the enzyme occurs at high levels in third instar females and is maintained at that level during fourth instar when gametogenesis, fertilization and chromosome imprinting occur. These results suggest a developmental stage‐specific modulation of levels of DNA methyltransferase. Assays with synthetic polymers showed that the enzyme can methylate not only polymers containing GpG but also those containing CpA and CpI.