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Cloning and sequencing of the human nucleolin cDNA
Author(s) -
Srivastava Meera,
Fleming Patrick J.,
Pollard Harvey B.,
Burns A.Lee
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80692-1
Subject(s) - nucleolin , complementary dna , cloning (programming) , computational biology , microbiology and biotechnology , biology , chemistry , genetics , gene , computer science , programming language , cytoplasm , nucleolus
A cDNA containing the entire coding region for human nucleolin has been isolated from a λ gt10 human retinal library using a bovine cDNA probe. The cDNA hybridized to a transcript of 3000 bases from fast‐dividing cells, as well as terminally differentiated tissues of several species. Translation of the nucleotide sequence revealed a long open reading frame which predicts a 707 amino acid protein with several distinct domains. These include repeating elements, four conserved RNA‐binding regions, a glycine‐rich carboxy‐terminal domain and sites for phosphorylation, glycosylation and dibasic cleavage. Human and bovine nucleolin exhibited more additions and/or substitutions of aspartate, glutamate and serine residues in the chromatin‐binding domains by comparison with the hamster and mouse nucleolins. These differences may be related to species‐specific functions in transcription.