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Structure of the α 1 subunit of horse Na,K‐ATPase gene
Author(s) -
Kano Itsu,
Nagai Fumiko,
Satoh Kanako,
Ushiyama Keiko,
Nakao Toshiko,
Kano Kazutaka
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80691-x
Subject(s) - microbiology and biotechnology , genomic dna , exon , gene , complementary dna , coding region , nucleic acid sequence , biology , scn3a , g alpha subunit , protein subunit , intron , genetics , peptide sequence , genomic library , amino acid , dna
Genomic DNA for Na,K‐ATPase α 1 subunit was obtained from libraries of horse kidney genomic DNA in Charon 4A and in EMBL3 bacteriophages by screening with the full sized cDNA probe of the α 1 subunit of rat Na,K‐ATPase as probe. The gene spans 30 kb and consists of 23 exons and 22 intervening sequences. Intron‐exon boundaries were analyzed. The protein‐coding nucleotide sequence encodes 1016 amino acids with an M r of 112 264. The putative amino acid sequence of horse α 1 is 96–97% homologous to those of other mammalian species.