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Identification of 1,4‐thiomorpholine‐3‐carboxylic acid (TMA) in normal human urine
Author(s) -
Matarese R.M.,
Solinas S.P.,
Montefoschi G.,
Ricci G.,
Cavallini D.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80688-x
Subject(s) - urine , chemistry , carboxylic acid , identification (biology) , chromatography , biochemistry , biology , ecology
The sulfur‐containing cyclic imino acid 1,4‐thiomorpholine‐3‐carboxylic acid (TMA) has been identified in normal human urine. After the enrichment procedure with ion‐exchange chromatography, the urine extracts were reacted with diazomethane. Gas‐liquid chromatography revealed the presence of two peaks with the same retention times exhibited by authentic TMA after the same derivatization. The two compounds have been identified by mass‐spectrometry as the monomethylated and dimethylated derivatives of TMA. This result represents the first indication of the occurrence of TMA in a mammalian sample.