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Induction of tumour hypoxia by a vasoactive agent A combined NMR and radiobiological study
Author(s) -
Dunn J.F.,
Frostick S.,
Adams G.E.,
Stratford I.J.,
Howells N.,
Hogan G.,
Radda G.K.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80655-6
Subject(s) - hydralazine , hypoxia (environmental) , vasoactive , chemistry , medicine , oxygen , organic chemistry , blood pressure
The effect of hydralazine treatment on 3 murine tumours (RIF‐1, KHT and 16/C) was monitored using 31 P‐NMR. Changes in the 31 P‐NMR spectrum are compared with measurements of radiobiological hypoxic fraction (RHF) in the RIF‐1 and KHT. Hydralazine is known to reduce temporarily blood flow in experimental tumours, and thus cause a transient increase in the RHF to 100% (in RIF‐1 and KHT). This correlates with a decline in energy status as measured by 31 P‐NMR (i.e. there was an increase in P i in all three tumours). Time‐course data from the RIF‐1 and KHT tumours show that maintenance of anaesthesia prolongs the hypoxia induced by hydralazine.