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Transcriptional activity is inducible in the cauliflower mosaic virus 35 S promoter engineered with the heat shock consensus sequence
Author(s) -
Pietrzak Maciej,
Burri Markus,
Herrero Juan-Jose,
Mosbach Klaus
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80648-9
Subject(s) - cauliflower mosaic virus , promoter , heat shock factor , promoter activity , transcription (linguistics) , microbiology and biotechnology , heat shock , biology , heat shock protein , gene , gene expression , genetics , hsp70 , transgene , genetically modified crops , linguistics , philosophy
Transcriptional activity of a strong constitutive plant promoter, the cauliflower mosaic virus (CaMV) 35 S promoter, could be modulated by addition of multiple synthetic oligonucleotides, carrying the heat shock promoter consensus sequence. The activity of this modified promoter increased up to 3‐fold under heat shock conditions. A similar construction using enhancerless 35 S promoter resulted in an engineered promoter, which showed an increased activity under both normal and heat conditions, without a significant induction by the heat shock. We did not observe a silencer effect of the heat shock elements in combination with either complete or enhancerless 35 S promoter, using transient expression assay to measure the transcription activity.