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Expression of high‐ and low‐affinity epidermal growth factor receptors in human hepatoma cell lines
Author(s) -
Clementi Massimo,
Festa Antonella,
Testa Ivano,
Bagnarelli Patrizia,
Devescovi Giulia,
Carloni Guido
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80645-3
Subject(s) - epidermal growth factor , receptor , cell culture , biology , cell surface receptor , microbiology and biotechnology , hepatitis b virus , epidermal growth factor receptor , gene expression , gene , virus , virology , biochemistry , genetics
Data are presented from a comparative research on expression of epidermal growth factor (EGF) receptors and response to EGF of six independently established cell lines derived from human hepatoma. These lines differ in terms of the degree of differentiation, presence of hepatitis B virus (HBV) DNA copies in integrated form and expression of HBV genes. Our results indicate differential expression of membrane EGF receptors and differential response to EGF under serum‐ and hormone‐free culture conditions. Furthermore, a significant difference in affinity could be detected between EGF receptors of the two highly dedifferentiated cell lines (HA22T/VGH and Li7A) whose replication is inhibited by EGF concentrations capable of stimulating more differentiated phenotypes.