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The constituents of storage granules in the dermal glands of Xenopus laevis Structure of a basic polypeptide deduced from cloned cDNA
Author(s) -
Berger Hans,
Kreil Günther
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80644-1
Subject(s) - xenopus , complementary dna , amino acid , signal peptide , cdna library , biology , microbiology and biotechnology , peptide sequence , homology (biology) , biochemistry , gene
Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino‐terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were isolated from a cDNA expression library prepared from skin of X. laevis . One cDNA encodes a preprotein with a typical signal sequence and a mature part of 187 amino acids. The protein shows internal homology at both the amino and carboxyl end. The latter part has a very high content of basic amino acids.