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Localization and characterization of the glycosylation site of human pancreatic elastase 1
Author(s) -
Wendorf Petra,
Geyer Rudolf,
Sziegoleit Andreas,
Linder Dietmar
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80640-4
Subject(s) - glycosylation , trypsin , elastase , pancreatic elastase , biochemistry , chemistry , pancreas , amino acid residue , peptide sequence , enzyme , gene
Crystalline elastase 1 from human pancreas was digested with trypsin. Two peptides, containing the potential N ‐glycosylation sites at Asn‐86 and Asn‐125, were isolated and analyzed by amino acid analysis, sequencing and carbohydrate component analysis. The results demonstrate that only Asn‐86 is glycosylated.