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Neuropeptide Y inhibits vasoactive intestinal peptide‐ and dopamine‐induced cyclic AMP formation in human Ewing's sarcoma WE‐68 cells
Author(s) -
van Valen F.,
Keck E.,
Jürgens H.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80639-8
Subject(s) - neuropeptide y receptor , vasoactive intestinal peptide , pertussis toxin , medicine , endocrinology , chemistry , adenylate kinase , neuropeptide , agonist , intracellular , dopamine , cyclase , g protein , biology , receptor , biochemistry , stimulation
Neuropeptide Y (NPY) regulation of intracellular cyclic AMP accumulation was studied in human Ewing's sarcoma cell line, WE‐68. NPY inhibited vasoactive intestinal peptide (VIP)‐ and dopamine‐stimulated but not basal cyclic AMP formation. The peptide effect was rapid (<2 min), concentration‐dependent with a half‐maximal effective concentration (EC 50 ) of 8 nM NPY, and maximal inhibition reaching 60–70% with 100 nM NPY. Prior exposure of WE‐68 cells to pertussis toxin completely abolished the inhibitory action of NPY. It is concluded that NPY attenuates agonist‐stimulated cyclic AMP formation in Ewing's sarcoma WE‐68 cells, and may do so via the inhibitory guanine nucleotide regulatory protein of adenylate cyclase.