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Exon‐intron organization, expression, and chromosomal localization of the human motilin gene
Author(s) -
Yano H.,
Seino Y.,
Fujita J.,
Yamada Y.,
Inagaki N.,
Takeda J.,
Bell G.I.,
Eddy R.L.,
Fan Y.-S.,
Byers M.G.,
Shows T.B.,
Imura H.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80633-7
Subject(s) - motilin , microbiology and biotechnology , exon , biology , complementary dna , intron , gene , southern blot , genetics , endocrinology
The human motilin gene has been isolated and characterized. The gene spans about 9 kilobase pairs (kb) and the 0.7 kb motilin mRNA is encoded by five exons. The 22‐amino‐acid motilin sequence is encoded by exons 2 and 3. The human motilin gene was mapped to the p21.2→p21.3 region of chromosome 6 by hybridization of the cloned cDNA to DNAs from a panel of reduced human‐mouse somatic cell hybrids and by in situ hybridization to human prometaphase chromosomes. RNA blotting using RNA prepared from various regions of the human gastrointestinal tract revealed high levels of motilin mRNA in duodenum and lower levels in the antrum of the stomach; motilin mRNA could not be detected by this procedure in the esophagus, cardia of the stomach, descending colon or gallbladder.