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Location of phosphorylation site and DNA‐binding site of a positive regulator, OmpR, involved in activation of the osmoregulatory genes of Escherichia coli
Author(s) -
Kato Masashi,
Aiba Hirofumi,
Tate Shin-ichi,
Nishimura Yoshifumi,
Mizuno Takeshi
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80617-9
Subject(s) - escherichia coli , phosphorylation , recombinant dna , gene , response regulator , biology , dna , binding site , bacterial outer membrane , promoter , regulator , dna binding protein , biochemistry , microbiology and biotechnology , transcription factor , gene expression , bacterial protein
The OmpR protein of Escherichia coli is a positive regulator involved in activation of the ompF and ompC genes which encode the major outer membrane proteins OmpF and OmpC, respectively. By employing recombinant DNA techniques, we isolated the N‐ and C‐terminal halves of the OmpR molecule. From the results of biochemical analyses of these fragments, it was concluded that the N‐terminal portion contains a site involved in phosphorylation by an OmpR‐specific protein kinase EnvZ, whereas the C‐terminal part possesses a DNA‐binding site for the ompC and ompF promoters.