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Oscillating enzyme‐bound NADH in glycolysis
Author(s) -
Yuan Z.,
Müller S.C.,
Hess B.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80615-5
Subject(s) - cofactor , saccharomyces cerevisiae , yeast , alcohol dehydrogenase , nad+ kinase , biochemistry , enzyme , absorbance , chemistry , cytoplasm , chromatography
In glycolyzing cell‐free cytoplasmic medium extracted from yeast Saccharomyces cerevisiae , the action spectrum of oscillation has an absorption maximum around 335 nm, nearly coinciding with that of the yeast alcohol dehydrogenase (ADH)‐NADH complex due to its bound NADH. Our approximate calculations based on the amount of this enzyme and coenzyme NADH present in the extract suggest that the ADH‐NADH complex alone can account for 90% or more of the total absorbance change.