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Inhibitors of arachidonic acid metabolism eliminate the increase in cytosolic free calcium induced by the mitogen concanavalin A in rat thymocytes
Author(s) -
Gukovskaya A.S.,
Pulido H.Arias,
Zinchenko V.P.,
Evtodienko Yu.V.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80583-6
Subject(s) - nordihydroguaiaretic acid , lipoxygenase , arachidonic acid , concanavalin a , chemistry , phospholipase a2 , metabolism , biochemistry , cyclooxygenase , calcium , cytosol , endocrinology , enzyme , biology , organic chemistry , in vitro
Using inhibitors of arachidonic acid (AA) metabolism, the possible involvement of AA products in the generation of [Ca 2+ ] i and the pH i rise induced by the mitogen concanavalin A (Con A) in rat thymocytes has been studied. The lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA, 10 μM) and the phospholipase A 2 inhibitor bromophenacyl bromide (10 μM) eliminated the [Ca 2+ ] i signal induced by Con A; the cyclooxygenase blocker indomethacin also inhibited it. However, neither NDGA nor indomethacin suppressed the pH i rise stimulated by Con A. Exogenous AA induced an increase in [Ca 2+ ] i but not in the pH i . These results indicate that AA metabolites, probably of the lipoxygenase pathway, take part in the generation of the [Ca 2+ ] i response to the mitogen. In contrast, they appear not to be involved in the pH i rise evoked by Con A.