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Expression of ricin B chain in Escherichia coli
Author(s) -
Hussain Khalid,
Bowler Christopher,
Roberts Lynne M.,
Lord J.Michael
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80568-x
Subject(s) - ricin , periplasmic space , recombinant dna , escherichia coli , signal peptide , plasmid , microbiology and biotechnology , chemistry , expression vector , peptide , dna , biology , biochemistry , toxin , gene
DNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN‐111‐ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form.