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Squalene epoxide cyclase and microemulsion
Author(s) -
Lee Kang Min,
Duriatti Albert,
Schuber Francis,
Biellmann Jean-François
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80560-5
Subject(s) - microemulsion , squalene , chemistry , cationic polymerization , cyclase , pulmonary surfactant , epoxide , chromatography , calcium , titration , nuclear chemistry , organic chemistry , biochemistry , enzyme , catalysis
Squalene epoxide cyclase was extracted from microsomal preparations of rat liver using anionic, cationic and non‐ionic microemulsions. The anionic microemulsion was the best with respect to protein solubilisation, extracted cyclase activity and stability of this activity over time. The activity assay showed cyclase activity to be higher in anionic microemulsion than in buffer in the presence of surfactant. Calcium chloride in the anionic microemulsion had a stabilising effect and less total protein seemed to be extracted.