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Evidence for the monomeric nature of thymosins
Author(s) -
Haritos A.A.,
Yialouris P.P.,
Heimer E.P.,
Felix A.M.,
Hannappel E.,
Rosemeyer M.A.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80547-2
Subject(s) - sedimentation equilibrium , ultracentrifuge , monomer , thymosin , size exclusion chromatography , chemistry , analytical ultracentrifugation , chromatography , biochemistry , organic chemistry , enzyme , polymer
According to gel‐filtration experiments, α‐ and β‐thymosins appear to form oligomers, which are 4‐5‐fold larger than the corresponding polypeptides. However, on analysis by sedimentation equilibrium ultracentrifugation, prothymosin α and thymosin β 4 showed relative molecular masses of 12 800 and 4600, which are close to the values calculated from their amino acid sequences, confirming their existence in solution as discrete monomeric entities.