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Interaction between bacteriophage T 4 coded gene 32 protein and poly(rA)
Author(s) -
Watanabe Fumiyuki
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80519-8
Subject(s) - cooperativity , polynucleotide , cooperative binding , chemistry , molecule , bacteriophage , gene , salt (chemistry) , binding site , biochemistry , escherichia coli , organic chemistry
The cooperative binding of T 4 gene 32 protein with polynucleotides, of which the quantitative aspects in the literature have not satisfied the requirements of thermodynamics, is studied by adopting a modified formula of the lattice theory. A moderate value is found for the cooperativity parameter ( q ∼ 200 at 0.2 M NaCl), which is weakly dependent on salt concentration. The cation effect on the binding suggests that the shielding of negative charges of the protein or a loose cation bridge between the bound protein molecules plays a role in the cooperative binding process.