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A 10 kDa polypeptide associated with the oxygen‐evolving complex of photosystem II has a putative C‐terminal non‐cleavable thylakoid transfer domain
Author(s) -
Webber Andrew N.,
Packman Leonard C.,
Gray John C.
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80517-4
Subject(s) - thylakoid , photosystem ii , chloroplast , biochemistry , peptide sequence , amino acid , oxygen evolving complex , biology , n terminus , biophysics , chemistry , photosynthesis , gene
The N‐terminal amino acid sequence of the 10 kDa polypeptide associated with the oxygen‐evolving complex of wheat photosystem II has been determined and shown to be homologous to the amino acid sequence of the product of the ST‐LS1 gene from potato. The N‐terminal sequence of the mature protein indicates that the polypeptide is synthesized with a 39 amino acid N‐terminal presequence which is similar to chloroplast import sequences but which lacks a hydrophobic domain for transfer of the protein across the thylakoid membrane. The mature polypeptide has a C‐terminal hydrophobic region which shows homology to the hydrophobic thylakoid transfer domain of other lumenal proteins and this hydrophobic region of the 10 kDa polypeptide is suggested to facilitate transfer of the protein across the thylakoid membrane.