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SecA protein is directly involved in protein secretion in Escherichia coli
Author(s) -
Kawasaki Hisashi,
Matsuyama Shin-ichi,
Sasaki Shoichi,
Akita Mitsuru,
Mizushima Shoji
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80516-2
Subject(s) - secretion , chromosomal translocation , secretory protein , escherichia coli , vesicle , mutant , cytoplasm , vesicle associated membrane protein 8 , biology , urea , plasmid , biochemistry , microbiology and biotechnology , membrane protein , chemistry , gene , membrane
A high‐expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secA ts mutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membrane.