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Cell surface fibronectin of mouse peritoneal macrophages
Author(s) -
Beppu Masatoshi,
Masa Hideo,
Kikugawa Kiyomi
Publication year - 1989
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(89)80505-8
Subject(s) - trypsinization , fibronectin , macrophage , chemistry , receptor , microbiology and biotechnology , fibronectins , cell surface receptor , cell , biology , biochemistry , trypsin , in vitro , enzyme
Fibronectin (FN) was detected on thioglycollate‐induced mouse peritoneal macrophages by binding the 125 I‐labeled F(ab′) 2 fragment of rabbit anti‐human plasma fibronectin. The cell surface fibronectin (sFN) was removed from the surface of the macrophage monolayer by limited trypsinization. After trypsinization, binding of 125 I‐labeled plasma fibronectin ( 125 I‐pFN) to the macrophage monolayer was increased, suggesting that the FN receptor covered with sFN was exposed by trypsinization without destroying the receptor activity. The amounts of saturation binding of 125 I‐pFN to the macrophage monolayers before and after trypsinization were about 2.4 and 6.3 μg per 10 6 cells, respectively, indicating that the macrophage monolayer has the capacity of binding 6.3 μg FN per 10 6 cells, and the FN receptor equivalent to about 4 μg pFN per 10 6 cells is covered with sFN.